OSTİM Technical University Library

Sepetiniz boş.

Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols.

Yazar:Till, Bradley J
Katkıda bulunan(lar):Jankowicz-Cieslak, Joanna | Huynh, Owen A | Beshir, Mayada M | Laport, Robert G | Hofinger, Bernhard J
Materyal türü: KonuKonuYayıncı: Cham : Springer International Publishing AG, 2015Telif hakkı tarihi: �2015Tanım: 1 online resource (42 pages)İçerik türü:text Ortam türü:computer Taşıyıcı türü: online resourceISBN: 9783319162591Tür/Form:Electronic books.Ek fiziksel biçimler:Print version:: Low-Cost Methods for Molecular Characterization of Mutant PlantsLOC classification: TP248.27.P55Çevrimiçi kaynaklar: Click to View
İçindekiler:
Intro -- Foreword -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues -- 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes -- References -- Chapter 2: Health and Safety Considerations -- 2.1 Guidelines -- 2.2 Preparation of a Home-Made Chemical Spill Kit -- Reference -- Chapter 3: Sample Collection and Storage -- 3.1 Background -- 3.2 Materials -- 3.3 Methods -- References -- Chapter 4: Low-Cost DNA Extraction -- 4.1 Materials -- 4.2 Methods -- 4.2.1 Preparation of Silica Powder DNA Binding Solution -- 4.2.2 Low-Cost Extraction of Genomic DNA -- 4.3 Alternative Buffers for DNA Extraction -- Reference -- Chapter 5: PCR Amplification for Low-Cost Mutation Discovery -- 5.1 Materials -- 5.2 Methods -- References -- Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- 6.1 Materials -- 6.2 Methods -- Reference -- Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl... -- 7.1 Materials -- 7.2 Methods -- 7.2.1 Enzyme Extraction -- 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume -- in 1.5-m... -- 7.2.3 Test of Mismatch Cleavage Activity -- Reference -- Chapter 8: Example Data -- 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method -- 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers -- 8.2.1 Summary -- 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method -- 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations.
8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract -- 8.5.1 Summary -- References -- Chapter 9: Conclusions.
Bu kütüphanenin etiketleri: Kütüphanedeki eser adı için etiket yok. Etiket eklemek için oturumu açın.
    Ortalama derecelendirme: 0.0 (0 oy)
Bu kayda ilişkin materyal yok

Intro -- Foreword -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues -- 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes -- References -- Chapter 2: Health and Safety Considerations -- 2.1 Guidelines -- 2.2 Preparation of a Home-Made Chemical Spill Kit -- Reference -- Chapter 3: Sample Collection and Storage -- 3.1 Background -- 3.2 Materials -- 3.3 Methods -- References -- Chapter 4: Low-Cost DNA Extraction -- 4.1 Materials -- 4.2 Methods -- 4.2.1 Preparation of Silica Powder DNA Binding Solution -- 4.2.2 Low-Cost Extraction of Genomic DNA -- 4.3 Alternative Buffers for DNA Extraction -- Reference -- Chapter 5: PCR Amplification for Low-Cost Mutation Discovery -- 5.1 Materials -- 5.2 Methods -- References -- Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- 6.1 Materials -- 6.2 Methods -- Reference -- Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl... -- 7.1 Materials -- 7.2 Methods -- 7.2.1 Enzyme Extraction -- 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume -- in 1.5-m... -- 7.2.3 Test of Mismatch Cleavage Activity -- Reference -- Chapter 8: Example Data -- 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method -- 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers -- 8.2.1 Summary -- 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method -- 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations.

8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract -- 8.5.1 Summary -- References -- Chapter 9: Conclusions.

Description based on publisher supplied metadata and other sources.

Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2022. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.

There are no comments on this title.

yorum yazmak için.

Ziyaretçi Sayısı

Destekleyen Koha